5AFP
Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with rhodopsin kinase peptide from Homo sapiens
Replaces: 4UYCExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Collection date | 2014-07-12 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.690, 93.690, 55.710 |
Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
Resolution | 47.860 - 2.300 |
R-factor | 0.22708 |
Rwork | 0.226 |
R-free | 0.25515 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2YOU |
RMSD bond length | 0.002 |
RMSD bond angle | 1.238 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 | 0.370 |
Number of reflections | 18488 | |
<I/σ(I)> | 15.8 | 2.2 |
Completeness [%] | 99.2 | 94.3 |
Redundancy | 3.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.12M ALCOHOLS (1,6-HEXANEDIOL; 1- BUTANOL;1,PROPANEDIOL(RACEMIC); 2-PROPONOL; 1, 4-BUTANEDIOL; 1,3- PROPANEDIOL); 0.1M BUFFER 2 (SODIUM HEPES; MOPS ACID PH 7.5); P550MME_P20K 30%. |