5AFO
Long Polar Fimbriae adhesin LpfD from the adherent invasive E. coli strain LF82
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.010, 372.130, 99.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.590 - 1.820 |
| R-factor | 0.16847 |
| Rwork | 0.167 |
| R-free | 0.19600 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 82152 | |
| <I/σ(I)> | 23.95 | 2.63 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 11.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS PH 8.0, 0.18 M SODIUM CITRATE, 20% PEG 3350 |
