5AFF

Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-30
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	60.087, 105.959, 147.234
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.256 - 3.398
R-factor	0.2391
Rwork	0.236
R-free	0.29980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4GMO AND 3U5E CHAIN J
RMSD bond length	0.007
RMSD bond angle	1.332
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.080	0.580
Number of reflections	13505
<I/σ(I)>	15.8	3.19
Completeness [%]	99.8	98.9
Redundancy	8.1	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M SODIUM ACETATE, 8% (V/V) PEG 4000