5AFE
Medium Resolution structure of the C-terminal family 65 Carbohydrate Binding Module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens with a bound xyloglucan heptasaccharide (XXXG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 58.960, 58.960, 117.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.660 - 2.600 |
R-factor | 0.25122 |
Rwork | 0.249 |
R-free | 0.30613 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ba6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.112 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.960 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.270 | 1.500 |
Number of reflections | 6535 | |
<I/σ(I)> | 2.5 | 0.6 |
Completeness [%] | 96.6 | 97.2 |
Redundancy | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 30%(W/V) PEG 4000 CO-CRYSTALLISED WITH 10 MM OF HEPTASACCHARIDE XXXG CRYOPROTECTAN WAS 30% GLYCEROL ADDED TO ABOVE CRYSTALLISATION BUFFER |