5AFB
Crystal structure of the Latrophilin3 Lectin and Olfactomedin Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2014-08-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07114, 1.0716 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.430, 96.650, 101.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.910 - 2.160 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.24520 |
Structure solution method | SIR |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | autoSHARP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.090 | 2.270 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.100 | 0.860 |
Number of reflections | 39860 | |
<I/σ(I)> | 10.2 | 1.7 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 3.49 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1 M MES BUFFER, 4M NACL, 0.6 M NON- DETERGENT SULFO-BETAINE NDSB195, PH 6.0 |