5AFA
Crystal structure of Laccase from Thermus thermophilus HB27 complexed with Ag, crystal of the holoenzyme soaked for 30 m in 5 mM AgNO3 at 278 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-16 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.706, 110.579, 96.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.346 - 2.193 |
| R-factor | 0.1484 |
| Rwork | 0.146 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.350 | 2.310 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.170 | 0.290 |
| Number of reflections | 25637 | |
| <I/σ(I)> | 5.1 | 3.6 |
| Completeness [%] | 98.2 | 88.5 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M HEPES PH 7.5, 70 % MPD BEFORE DATA COLLECTION THE CRYSTAL WAS SOAKED FOR 30 MINUTES IN 5 MM AGNO3 |
