5AF6
Structure of Lys33-linked diUb bound to Trabid NZF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-06-28 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.384, 126.512, 78.092 |
| Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
| Resolution | 38.591 - 3.400 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1UBQ AND 2WWZ |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.590 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.110 | 0.560 |
| Number of reflections | 12855 | |
| <I/σ(I)> | 6.6 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG 550 MME, 10% PEG 20K, 0.12 M NA OXAMATE, 0.12 M NAF, 0.12 M NA CITRATE, 0.12 M NA/K TARTRATE 0.1 M MES/IMIDAZOLE PH 6.5 |
