5AF5
Structure of Lys33-linked triUb S.G. P 212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-06-28 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.420, 42.480, 50.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.621 - 1.680 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.748 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.510 | 1.720 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.050 | 0.780 |
Number of reflections | 7403 | |
<I/σ(I)> | 19.9 | 2.7 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.3 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 10% PEG 20K, 20% PEG 550 MME, 0.02 M D-GLUCOSE, 0.02M D-MANNOSE, 0.02M D-GALACTOSE, 0.02 M L-FRUCTOSE, 0.02 M D-XYLOSE, 0.02 M N-ACETYL-D-GLUCOSAMINE, 100 MM TRIS/BICINE PH 8.5 |