5AF1
Crystal structure of Candida albicans Mep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 3 |
| Unit cell lengths | 100.151, 100.151, 109.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.582 - 1.639 |
| R-factor | 0.1367 |
| Rwork | 0.136 |
| R-free | 0.15910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.040 | 0.690 |
| Number of reflections | 151150 | |
| <I/σ(I)> | 20 | 2 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 5.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES PH 6 0.2 M LITHIUM SULPHATE 20% PEG400 |
