5AEZ
Crystal structure of Candida albicans Mep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.880, 104.880, 160.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.702 - 1.470 |
| R-factor | 0.1376 |
| Rwork | 0.137 |
| R-free | 0.14990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.070 | 0.610 |
| Number of reflections | 112103 | |
| <I/σ(I)> | 11.5 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.04 M TRIS PH 8.0 0.04 M NACL 27% PEG350 MME |
