5AEM
Structure of t131 N-terminal TPR array
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 105.130, 105.130, 98.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.338 - 3.400 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24450 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.340 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.080 | 0.590 |
| Number of reflections | 14895 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.9 | 0.1 M BICINE PH 8.9 0.85 M MGCL2 |
