5AEC
Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.
Replaces: 2WGKExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.963, 93.317, 161.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.950 - 1.930 |
| R-factor | 0.17388 |
| Rwork | 0.171 |
| R-free | 0.22139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1luc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.495 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.440 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 62419 | |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 98.3 | 88.3 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM PIPES PH 6.5, 50 % SATURATION AMMONIUM SULPHATE |
