5AE9
Crystal structure of mouse PI3 kinase delta in complex with GSK2292767
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.300, 64.620, 116.370 |
| Unit cell angles | 90.00, 103.32, 90.00 |
Refinement procedure
| Resolution | 47.440 - 2.440 |
| R-factor | 0.19 |
| Rwork | 0.186 |
| R-free | 0.26330 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 2.450 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 37812 | |
| <I/σ(I)> | 13 | 2.1 |
| Completeness [%] | 98.1 | 83.1 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
