5AE3
Ether Lipid-Generating Enzyme AGPS in complex with antimycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23 |
| Synchrotron site | ESRF |
| Beamline | ID23 |
| Temperature [K] | 100 |
| Collection date | 2014-04-15 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.159, 98.850, 107.722 |
| Unit cell angles | 90.80, 90.50, 94.79 |
Refinement procedure
| Resolution | 107.710 - 2.180 |
| R-factor | 0.19209 |
| Rwork | 0.191 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bby |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.790 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 123594 | |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 94.2 | 93.1 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PH 7.5 |
