5ACL
Mcg immunoglobulin variable domain with sulfasalazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Collection date | 2015-03-09 |
| Detector | RIGAKU HCT |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 31.860, 74.610, 39.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.320 - 1.490 |
| R-factor | 0.1597 |
| Rwork | 0.158 |
| R-free | 0.18742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4unu |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.037 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 15567 | |
| <I/σ(I)> | 19 | 4 |
| Completeness [%] | 98.0 | 83 |
| Redundancy | 6.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M TRI-POTASSIUM CITRATE, 2.2 M (NH4)2SO4, 0.5 M SULFASALAZINE, pH 8.5 |
