5ACG
X-ray Structure of LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 125.240, 125.240, 125.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.910 |
| R-factor | 0.17921 |
| Rwork | 0.178 |
| R-free | 0.20846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eir |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.220 | 1.050 |
| Number of reflections | 26532 | |
| <I/σ(I)> | 7.38 | 1.64 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 5.4 | 5.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 3.9M SODIUM CHLORIDE, 100MM CITRIC ACID PH4.0, pH 5.5 |
