5ABS
CRYSTAL STRUCTURE OF THE C-TERMINAL COILED-COIL DOMAIN OF CIN85 IN SPACE GROUP P321
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 40.968, 40.968, 90.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.020 - 1.740 |
| R-factor | 0.24796 |
| Rwork | 0.244 |
| R-free | 0.28327 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.926 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | SHELX (CDE) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.020 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.100 | 0.920 |
| Number of reflections | 9541 | |
| <I/σ(I)> | 15.48 | 1.09 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 17.9 | 16.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.05M ZN ACETATE, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 7 |
