5ABR
Structure of FeSI protein from Azotobacter vinelandii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-02 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.040, 60.100, 45.510 |
Unit cell angles | 90.00, 109.37, 90.00 |
Refinement procedure
Resolution | 42.934 - 2.110 |
R-factor | 0.18 |
Rwork | 0.177 |
R-free | 0.23070 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.024 |
RMSD bond angle | 2.373 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.930 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.130 | 0.530 |
Number of reflections | 11386 | |
<I/σ(I)> | 8.1 | 2.4 |
Completeness [%] | 98.7 | 97.9 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100 MM HEPES PH 7.5, 77% V/V MPD |