5AB8
High resolution X-ray structure of the N-terminal truncated form (residues 1-11) of Mycobacterium tuberculosis HbN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 43.160, 43.160, 212.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.070 - 1.530 |
| R-factor | 0.14384 |
| Rwork | 0.140 |
| R-free | 0.20917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1idr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.508 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.230 | 1.610 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 18944 | |
| <I/σ(I)> | 15.7 | 5.8 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 15.5 | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 277 | 2.0 M NA-FORMATE, 0.1 M NA-ACETATE PH 4.6, T=277 K |
