5AB7
Crystal structure of Trypanosoma brucei SCP2-thiolase like protein (TbSLP) in complex with malonyl-CoA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-20 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.546, 66.404, 159.834 |
Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
Resolution | 29.400 - 2.300 |
R-factor | 0.23655 |
Rwork | 0.236 |
R-free | 0.24887 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.380 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.130 | 0.710 |
Number of reflections | 104486 | |
<I/σ(I)> | 7.5 | 1.9 |
Completeness [%] | 95.3 | 93.7 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 100 MM SODIUM ACETATE (PH 4.6), 2.0 M AMMONIUM SULPHATE |