5AB5
Crystal structure of Trypanosoma brucei SCP2-thiolase like protein (TbSLP) form-II.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 |
| Unit cell lengths | 66.689, 66.689, 157.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.877 - 2.000 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.880 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 1.250 |
| Number of reflections | 52628 | |
| <I/σ(I)> | 8.3 | 1.3 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE (PH 4.6), 2.0 M AMMONIUM SULPHATE |
