5AAR
Structure of the ankyrin domain of an Arabidopsis Thaliana potassium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.921, 85.570, 65.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.785 - 1.870 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xeh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.790 | 1.890 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.080 | 0.900 |
| Number of reflections | 16865 | |
| <I/σ(I)> | 13.8 | 1.1 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 8.5, 25% PEG 4000, 0.3 M MGCL2 |
