5AAO
Crystal structure of fluorogen-activating designed ankyrin repeat protein (DARPin) dimer in complex with malachite green
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-25 |
Detector | DECTRIS PILATUS 2M-F |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.512, 109.754, 100.301 |
Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
Resolution | 19.951 - 2.600 |
R-factor | 0.2159 |
Rwork | 0.213 |
R-free | 0.26220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1svx |
RMSD bond length | 0.001 |
RMSD bond angle | 0.453 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.273 | |
Number of reflections | 62041 | |
<I/σ(I)> | 6.6 | 0.86 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 2.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% (W/V) PEG3350, 0.2 M SODIUM MALONATE, PH 5.0 |