5AA2
Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAM-pentapeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.585, 135.652, 137.459 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 48.261 - 2.800 |
| R-factor | 0.1709 |
| Rwork | 0.167 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 1.500 |
| Number of reflections | 59073 | |
| <I/σ(I)> | 5.1 | 0.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
