5A9Y
Structure of ppGpp BipA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 244.310, 244.310, 244.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.910 - 4.000 |
| R-factor | 0.259 |
| Rwork | 0.254 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BIPA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 4.240 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.160 | |
| Number of reflections | 10792 | |
| <I/σ(I)> | 14 | 0.82 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 18.2 | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG3350, 0.2 M SODIUM FLUORIDE |
