5A9Y
Structure of ppGpp BipA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 244.310, 244.310, 244.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.910 - 4.000 |
R-factor | 0.259 |
Rwork | 0.254 |
R-free | 0.32100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BIPA |
RMSD bond length | 0.010 |
RMSD bond angle | 1.441 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 4.240 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.160 | |
Number of reflections | 10792 | |
<I/σ(I)> | 14 | 0.82 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 18.2 | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% (W/V) PEG3350, 0.2 M SODIUM FLUORIDE |