5A9H
Crystal structure of the extracellular domain of PepT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 43.070, 43.070, 220.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.460 - 2.060 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.460 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.070 | 0.670 |
| Number of reflections | 13761 | |
| <I/σ(I)> | 11.5 | 2.3 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.2 M CSCL2, 15 % PEG 3350 AT 20 DEGREES CELCIUS |
