5A9G
Manganese Superoxide Dismutase from Sphingobacterium sp. T2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.700, 58.940, 75.710 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 36.600 - 1.350 |
| R-factor | 0.13838 |
| Rwork | 0.138 |
| R-free | 0.15663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.692 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.410 |
| Number of reflections | 87001 | |
| <I/σ(I)> | 18.2 | 3.5 |
| Completeness [%] | 96.5 | 79 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% (W/V) PEG 3350, 0.2 M DI-AMMONIUM HYDROGEN CITRATE, pH 8.5 |
