5A9D
Crystal structure of the extracellular domain of PepT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-22 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.480, 70.330, 111.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.620 - 2.100 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 2.190 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.800 |
Number of reflections | 25179 | |
<I/σ(I)> | 16.4 | 2.7 |
Completeness [%] | 99.5 | 99 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 % PEG 6000, 0.1 M MES PH 6.0, 0.2 M AMMONIUM CHLORIDE, AT 10 MG.ML-1 AND A 4 DEGREES CELCIUS USING SITTING DROP VAPOUR DIFFUSION PLATES. |