5A9D
Crystal structure of the extracellular domain of PepT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.480, 70.330, 111.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.620 - 2.100 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.800 |
| Number of reflections | 25179 | |
| <I/σ(I)> | 16.4 | 2.7 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 % PEG 6000, 0.1 M MES PH 6.0, 0.2 M AMMONIUM CHLORIDE, AT 10 MG.ML-1 AND A 4 DEGREES CELCIUS USING SITTING DROP VAPOUR DIFFUSION PLATES. |
