5A9C

Crystal structure of Antheraea mylitta CPV4 polyhedra base domain deleted mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 3
Unit cell lengths	104.330, 104.330, 104.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.883 - 1.710
R-factor	0.1869
Rwork	0.185
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CPV4 POLYHEDRA TYPE 2 STRUCTURE
RMSD bond length	0.003
RMSD bond angle	0.735
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.750
High resolution limit [Å]	1.710	1.710
Rmerge	0.190
Number of reflections	19985
<I/σ(I)>	5	0.6
Completeness [%]	97.3	88.9
Redundancy	4.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.5