5A9A
Crystal structure of Simulium ubiquitum CPV20 polyhedra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.360, 103.360, 103.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.197 - 1.822 |
| R-factor | 0.1583 |
| Rwork | 0.157 |
| R-free | 0.18990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODELS OF POLYHEDRA STRUCTURE OF CPV5 CPV4 CPV1 CPV14 CPV15 AND CPV19 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.827 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.700 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.340 | |
| Number of reflections | 16582 | |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 10 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
