5A95
Crystal structure of beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide A, form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.048, 60.384, 130.326 |
| Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
| Resolution | 45.440 - 1.350 |
| R-factor | 0.13071 |
| Rwork | 0.129 |
| R-free | 0.15610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a8n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.630 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 237302 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% (W/V) PEG 4000, 0.1 M TRIS BUFFER PH 7.5, 0.2 M MGCL2 |
