5A94
Crystal structure of beta-Glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide A, form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.643, 132.753, 131.352 |
| Unit cell angles | 90.00, 133.78, 90.00 |
Refinement procedure
| Resolution | 47.420 - 1.990 |
| R-factor | 0.1795 |
| Rwork | 0.179 |
| R-free | 0.19038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WWPDB ENTRY 5A8N |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 157114 | |
| <I/σ(I)> | 6.6 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% (W/V) PEG 4000, 0.1 M TRIS BUFFER PH 7.5, 0.2 M MGCL2 |
