5A8Q
Crystal structure beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide A obtained by soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 143.702, 143.702, 143.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 1.900 |
| R-factor | 0.13886 |
| Rwork | 0.138 |
| R-free | 0.16098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a8o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 40497 | |
| <I/σ(I)> | 19.5 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM CACODYLATE BUFFER PH 6.0, 25% (W/V) PEG 8000 |
