5A8P
Crystal structure beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-31 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 144.382, 144.382, 144.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.700 - 2.200 |
R-factor | 0.15351 |
Rwork | 0.152 |
R-free | 0.18955 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WWPDB ENTRY 5A8O |
RMSD bond length | 0.007 |
RMSD bond angle | 1.138 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.130 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.160 | 0.770 |
Number of reflections | 26553 | |
<I/σ(I)> | 13.3 | 3 |
Completeness [%] | 99.6 | 100 |
Redundancy | 9.9 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM CACODYLATE BUFFER PH 6.0, 25% (W/V) PEG 8000 |