5A8O

Crystal structure of beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with cellotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Collection date	2013-05-31
Detector	DECTRIS P
Spacegroup name	P 41 3 2
Unit cell lengths	143.773, 143.773, 143.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 2.300
R-factor	0.15067
Rwork	0.149
R-free	0.17282
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a8n
RMSD bond length	0.007
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.580
Number of reflections	23212
<I/σ(I)>	17.5	4
Completeness [%]	100.0	100
Redundancy	9.5	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM CACODYLATE BUFFER PH 6.0, 25% (W/V) PEG 8000