5A8J
Crystal structure of the ArnB paralog VWA2 from Sulfolobus acidocaldarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-10 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.640, 74.280, 145.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.658 - 1.460 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.20130 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.340 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CCP4 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.660 | 1.540 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.050 | 0.490 |
Number of reflections | 65094 | |
<I/σ(I)> | 17.5 | 3.5 |
Completeness [%] | 99.2 | 97.7 |
Redundancy | 5.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | RESERVOIR BUFFER: 0.1 M SODIUMCITRATE PH 5.5; 2.5 M AMMONIUMSULFATE; PROTEIN BUFFER: 20 MM HEPES PH 7.0; 100 MM KCL, 2.7 MG/ML PROTEIN IN PROTEIN BUFFER; MIX RESERVOIR AND PROTEIN 1:1 |