5A8B
Structure of a parallel dimer of the aureochrome 1a LOV domain from Phaeodactylum tricornutum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.391, 75.550, 77.664 |
| Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
| Resolution | 38.700 - 2.791 |
| R-factor | 0.1733 |
| Rwork | 0.170 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ue6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.970 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.120 | 0.300 |
| Number of reflections | 16065 | |
| <I/σ(I)> | 8.5 | 308 |
| Completeness [%] | 98.9 | 94.3 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24%(W/V) PEG1500, 20%(V/V) GLYCEROL, pH 8 |
