5A89
Crystal structure of the riboflavin kinase module of FAD synthetase from Corynebacterium ammoniagenes in complex with FMN and ADP(P 21 21 21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.400, 70.480, 100.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.620 - 1.650 |
R-factor | 0.14577 |
Rwork | 0.143 |
R-free | 0.17551 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x0k |
RMSD bond length | 0.015 |
RMSD bond angle | 1.772 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.240 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.050 | 0.160 |
Number of reflections | 36054 | |
<I/σ(I)> | 18.5 | 5.1 |
Completeness [%] | 95.7 | 76.6 |
Redundancy | 5.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 26-30% PEG 4000, 200 MM LI2SO4 AND 100 MM SODIUM ACETATE PH 5.0 AND 100 MM NAI |