5A7Z
Crystal structure of Sulfolobus acidocaldarius Trm10 at 2.1 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.770, 46.320, 138.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.110 - 2.100 |
| R-factor | 0.22594 |
| Rwork | 0.224 |
| R-free | 0.26413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a7t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.010 | 0.120 |
| Number of reflections | 16213 | |
| <I/σ(I)> | 9.23 | 1.74 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 8.6 | 4.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 2 % DIOXANE, 0.05 M BICINE PH 9, 15 % PEG20K |
