5A7Y
Crystal structure of Sulfolobus acidocaldarius Trm10 in complex with S-adenosylhomocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-01 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.140, 101.090, 66.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.090 - 2.500 |
| R-factor | 0.23713 |
| Rwork | 0.234 |
| R-free | 0.28923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a7t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.492 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.010 | 0.160 |
| Number of reflections | 22754 | |
| <I/σ(I)> | 13.26 | 1.56 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.4 M (NH4)2SO4, 0.1 M SODIUM ACETATE PH 4.5, 15-20 % PEG2000MME |
