5A7T
Crystal structure of Sulfolobus acidocaldarius Trm10 at 2.4 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.730, 46.400, 139.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.010 - 2.400 |
| R-factor | 0.18239 |
| Rwork | 0.179 |
| R-free | 0.25001 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.010 | 0.100 |
| Number of reflections | 11139 | |
| <I/σ(I)> | 10.16 | 1.74 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.7 | 4.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8 % ETHYLENE GLYCOL, 0.1 M HEPES PH 7.5, 10 % PEG8K |
