5A7E
Crystallographic Structural Determination of a Trigonal Laccase from Coriolopsis Gallica (CgL) to 1.5 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2015-05-30 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 45.742, 56.406, 59.173 |
Unit cell angles | 75.67, 73.03, 76.49 |
Refinement procedure
Resolution | 43.082 - 1.500 |
R-factor | 0.1643 |
Rwork | 0.163 |
R-free | 0.18290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a2g |
RMSD bond length | 0.029 |
RMSD bond angle | 2.025 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.080 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.440 |
Number of reflections | 81678 | |
<I/σ(I)> | 15.8 | 4.1 |
Completeness [%] | 94.4 | 92 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.17 M AMMONIUM SULPHATE, 25.5% PEG 8000, 15% GLYCEROL |