5A7E
Crystallographic Structural Determination of a Trigonal Laccase from Coriolopsis Gallica (CgL) to 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.742, 56.406, 59.173 |
| Unit cell angles | 75.67, 73.03, 76.49 |
Refinement procedure
| Resolution | 43.082 - 1.500 |
| R-factor | 0.1643 |
| Rwork | 0.163 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2g |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.025 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.080 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 81678 | |
| <I/σ(I)> | 15.8 | 4.1 |
| Completeness [%] | 94.4 | 92 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.17 M AMMONIUM SULPHATE, 25.5% PEG 8000, 15% GLYCEROL |
