5A7A
Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.3 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 297 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.1 |
| Software | EMAN,IMAGIC,SPIDER |
