5A76
KSHV LANA (ORF73) C-terminal domain, open non-ring conformation: orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 200.224, 200.224, 83.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 141.580 - 3.800 |
| R-factor | 0.22347 |
| Rwork | 0.221 |
| R-free | 0.26908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k2j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 4.250 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.280 | 0.590 |
| Number of reflections | 16786 | |
| <I/σ(I)> | 5.2 | 3.2 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3 | 100 MM CITRIC ACID, PH 3.0, 1200 MM MAGNESIUM CHLORIDE. |
