5A6S
Crystal structure of the CTP1L endolysin reveals how its activity is regulated by a secondary translation product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 136.197, 136.197, 56.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.900 |
| R-factor | 0.16412 |
| Rwork | 0.162 |
| R-free | 0.20468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1JFX AND 2NW0 HYBRID MODEL |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.150 | 0.750 |
| Number of reflections | 40146 | |
| <I/σ(I)> | 9.6 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5-10 % PEG 8000, 20 MM TRIS PH 8.0 |
