5A6R
Crystal structure of the BTB domain of human KCTD17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-12 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 285.743, 285.743, 285.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.155 - 2.850 |
| R-factor | 0.2213 |
| Rwork | 0.220 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.150 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 1.500 |
| Number of reflections | 23906 | |
| <I/σ(I)> | 17.1 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18% MPD -- 0.1M TRIS PH 8.5 |
