5A6L
High resolution structure of the thermostable glucuronoxylan endo-Beta-1, 4-xylanase, CtXyn30A, from Clostridium thermocellum with two xylobiose units bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.133, 87.249, 60.027 |
| Unit cell angles | 90.00, 114.68, 90.00 |
Refinement procedure
| Resolution | 54.540 - 1.800 |
| R-factor | 0.13712 |
| Rwork | 0.136 |
| R-free | 0.16242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBE ENTRY 4UQE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.763 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.250 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.280 | 1.490 |
| Number of reflections | 43517 | |
| <I/σ(I)> | 17.5 | 9.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM PHOSPHATE |
