5A6D
Proliferating Cell Nuclear Antigen, PCNA, from Thermococcus gammatolerans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 |
| Unit cell lengths | 92.669, 92.669, 63.632 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.861 - 2.800 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lx1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.508 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 15022 | |
| <I/σ(I)> | 12.1 | 1.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 100 MM CITRATE BUFFER PH 5.2 2.8 M AMMONIUM SULPHATE 7.5% MPD |
