5A66
Crystal structure of AtTTM3 in complex with tripolyphosphate and manganese ion (form A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-20 |
| Detector | DECTRIS PILATUS |
| Wavelength(s) | 1.000000, 1.802090 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 136.273, 136.273, 145.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.180 - 2.050 |
| R-factor | 0.18549 |
| Rwork | 0.182 |
| R-free | 0.21963 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.180 | 2.170 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.740 |
| Number of reflections | 42478 | |
| <I/σ(I)> | 19.13 | 2.2 |
| Completeness [%] | 98.0 | 88.1 |
| Redundancy | 11.73 | 6.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 2.5 M NACL, 0.1 M SODIUM CITRATE PH 5.0 |
