5A5V
A complex of the synthetic siderophore analogue Fe(III)-6-LICAM with the CeuE periplasmic protein from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.370, 66.080, 68.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.970 - 2.040 |
| R-factor | 0.21618 |
| Rwork | 0.213 |
| R-free | 0.26528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1j |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.635 |
| Data reduction software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.260 | 1.240 |
| Number of reflections | 18467 | |
| <I/σ(I)> | 9.6 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M PCB BUFFER, PH 7, 25% PEG 1500 |
